缅北强奸

缅北强奸's Medicine by Design invests $1 million to advance new ideas in regenerative medicine

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缅北强奸's Amy Wong, a scientist working in developmental and stem cell biology at SickKids Hospital, is overseeing one of seven projects at 缅北强奸 and its partner hospitals supported by the 2020 New Ideas Awards聽and聽Seed Fund聽awards (photo by Dodge Baena)

Patients with cystic fibrosis experience recurrent lung infections that eventually destroy their airways, shortening their average life expectancy to 50 years in Canada. Current drug treatments, which target a malfunctioning pathway in cells that causes the infections, are costly and have varying effectiveness.

Now, with funding from Medicine by Design, a researcher at the Hospital for Sick Children (SickKids) is combining stem cells, gene editing and computational modelling to try to hijack an alternative cell pathway in the hopes of restoring lung function in these patients.

鈥淚f successful, our study will be the first to provide proof-of-concept that this alternative approach to treating cystic fibrosis is effective,鈥 says Amy Wong, a scientist working in developmental and stem cell biology at SickKids who is also an assistant professor in the department of laboratory medicine and pathobiology in the University of Toronto鈥檚 Temerty Faculty of Medicine.

Wong鈥檚 project is one of seven across 缅北强奸 and its affiliated hospitals that have been awarded 2020 New Ideas Awards and Seed Fund awards from Medicine by Design. Through a $1 million investment, Medicine by Design is supporting research aimed at advancing new concepts expected to be important to regenerative medicine in the coming years. The funded projects will have potential impacts in diseases and conditions such as vision loss, amyotrophic lateral sclerosis (ALS), intestinal disease in premature babies and more.

鈥淪upporting novel strategies and approaches is crucial to moving regenerative medicine into the future,鈥 says Michael Sefton, executive director of Medicine by Design and a at 缅北强奸鈥檚 Institute of Biomedical Engineering and the department of chemical engineering & applied chemistry in the Faculty of Applied Science & Engineering.

鈥淥ur 2020 New Ideas project portfolio integrates mathematical modelling, physics and computational biology with stem cell biology and biomedical engineering, and strengthens engagement with clinicians who are key to translating our research into patient impact. We are particularly delighted this year to support so many outstanding early-career researchers, who will ensure Toronto remains a global leader in regenerative medicine for years to come.鈥

Wong is one of three investigators to receive a 2020 New Ideas Award, which is valued at $100,000 per year for up to two years. Four additional projects were selected for Seed Fund Awards of $100,000 each for one year to further develop their potential.

Medicine by Design selected the funded projects from among 36 short-listed proposals, which were evaluated and ranked through an external peer review process. Applications were submitted by clinicians and researchers at 缅北强奸 and its affiliated hospitals from a wide range of disciplines including biochemistry, biomedical engineering, developmental and stem cell biology, immunology, neuroscience and surgery.

Medicine by Design builds on decades of made-in-Canada excellence in regenerative medicine dating back to the discovery of stem cells in the early 1960s by Toronto researchers James Till and  Ernest McCulloch. Regenerative medicine uses stem cells to replace diseased tissues and organs, creating therapies in which cells are the biological product. It can also mean triggering stem cells that are already present in the human body to repair damaged tissues or to modulate immune responses. Increasingly, regenerative medicine researchers are using a stem cell lens to identify critical interactions or defects that prepare the ground for disease, paving the way for new approaches to preventing disease before it starts. Medicine by Design is made possible thanks in part to a $114-million grant from the Canada First Research Excellence Fund.

New Ideas Awards

Current cystic fibrosis drug treatments target a genetic mutation that causes epithelial cells, which line the airway and act as a barrier against viruses, to function improperly. The mutation affects the function of an important ion channel in cells, called CFTR, which helps to maintain the right balance of fluid in the airways. Poor function causes mucosal obstructions in the airways and prevents clearance of foreign pathogens, which leads to chronic infections and ultimately destroys airway tissue.

In her project, Wong will explore an alternative ion channel in the epithelial cells to determine if it can be hijacked and used to compensate for the lack of function caused by the mutant CFTR. The research will be conducted using a combination of stem cell-derived lung models, gene editing and computational modelling.

Wong鈥檚 project builds on decades of cystic fibrosis research at SickKids, where the cystic fibrosis gene was first identified 30 years ago.

鈥淭o date, more than 2,000 mutations in the cystic fibrosis gene have been identified,鈥 says Wong. 鈥淪ickKids scientists and 缅北强奸 researchers have become the epicentre of incredible cystic fibrosis research to understand how this disease works at the genetic and molecular level.鈥

Wong says that, while the idea of targeting an alternative pathway is not necessarily ground-breaking on its own, it鈥檚 the array of tools now available that makes the idea a potential game changer.

鈥淲e have access to an incredible resource of primary cells and stem cells from more than 100 individuals with cystic fibrosis harbouring various mutations.鈥 Wong says. 鈥淥ur lab has developed human lung models from stem cells that can be used to model lung disease such as cystic fibrosis. And with new advanced tools in single-cell genomics and gene-editing, coupled with key collaborations for computational modelling, we are poised to find new therapeutic targets for cystic fibrosis.鈥

Leo Chou, an assistant professor at the Institute of Biomedical Engineering, and Hyun Kate Lee, an assistant professor in the department of biochemistry in the Temerty Faculty of Medicine 鈥 both Medicine by Design New Investigators 鈥 are also leading 2020 New Ideas projects.

Chou, along with co-investigators Julie Lefebvre, a scientist at SickKids and 缅北强奸 assistant professor of molecular genetics, and Valerie Wallace, a senior scientist at the Krembil Research Institute, University Health Network and a 缅北强奸 professor of laboratory medicine and pathobiology and ophthalmology, will focus on cell transplantation in the retina, a process that has demonstrated encouraging pre-clinical results such as partial vision restoration in several animal disease models.

Recent research had demonstrated that this restoration is a result of the transfer of proteins 鈥 complex molecules required for the structure, function and regulation of the body鈥檚 tissues 鈥 between host tissue and donor cells. But the scope of that transfer process is not well understood. Chou鈥檚 project will develop an imaging approach to detect the transfer of mRNA molecules between host and donor cells. The outcomes from this project will inform the future design of cell transplantation therapies and lead to novel methods to deliver therapeutics. This project could improve therapies for retinal diseases and visual impairments, and inform strategies for other degenerative disorders.

Lee and co-investigator Penney Gilbert, an associate professor at the Institute of Biomedical Engineering, will look at a common but not well-understood structure called the neuromuscular junction (NMJ), which mediates communication between neurons and muscles throughout the body. Defects in NMJ integrity and function underlie fatal diseases such as ALS. NMJ diseases, which affect more than 500,000 people globally, lack effective treatments. This project will use stem cells derived from reprogrammed skin cells of healthy people to develop NMJs in culture. Through high-resolution imaging, the healthy human NMJs will be studied both on their own and along with NMJs built from ALS patient cells. Through this work, the research team aims to identify genes to target to improve the health of NMJs, which could eventually help prevent or delay NMJ degeneration and even promote regeneration.

Seed Fund Awards

Michael Garton, an assistant professor at the Institute of Biomedical Engineering, has received a Seed Fund award to tackle the challenge of translating the genetic tools of synthetic biology 鈥 an area of research that aims to create or redesign biological components using engineering methods 鈥 into effective medical therapies against a number of diseases.

But they are difficult to translate into human therapies, Garton says, because the body鈥檚 T-cells 鈥 immune cells that detect and destroy cells containing foreign material 鈥 will identify these tools as foreign and destroy them.

Instead of switching off the T-cells, Garton鈥檚 goal is to use computational modelling and high-throughput screening to selectively turn off the body鈥檚 foreign antigen display system so the immune system will still respond to foreign invaders when necessary, but allow cells containing synthetic tools to survive. If successful, this approach could enable a new generation of synthetic biology-enhanced cell therapies for a range of diseases.

鈥淢edicine by Design funding will help to facilitate the integration of synthetic biology and regenerative medicine and aid the development of cell-based therapies that perform better than nature,鈥 says Garton.

Other Seed Fund projects will encompass research in repairing the heart after paediatric cardiac surgery, treating an intestinal emergency in premature babies and creating a database for cell lineage paths.

John Parkinson, a senior scientist at SickKids and a 缅北强奸 professor of biochemistry and molecular genetics, along with co-investigators Jason Maynes, Wasser Chair in Anesthesia and Pain Medicine at SickKids and a 缅北强奸 associate professor of anesthesiology and biochemistry, and William Navarre, an associate professor in the department of molecular genetics, will investigate manipulating the microbiome, or community of microorganisms in the gut, to improve cardiac repair in post-operative treatment of a congenital heart disorder. Through a process that will identify prebiotics in breast milk that help enhance the production of molecules that research has shown can aid cardiac repair, the team will organize both observational (how disease alters the microbiome) and interventional (how the microbiome alters the disease) multi-site trials, which will provide the opportunity to immediately translate findings into changes in patient care regimens and improve outcomes.

Clinicians Agostino Pierro, a surgeon at the Division of General and Thoracic Surgery at SickKids and a 缅北强奸 professor of surgery and physiology, and Philip Sherman, a senior scientist and gastroenterologist at the Division of Gastroenterology, Hepatology and Nutrition at SickKids and 缅北强奸 professor of dentistry, pediatrics and laboratory medicine and pathobiology, have proposed a novel way of enhancing gut repair for a common intestinal emergency in premature babies, called necrotizing enterocolitis (NEC). A leading cause of death for these infants, NEC causes complications such as blindness, intellectual disability, repeat hospitalizations and gut damage even in those that survive. This project will look at whether intestinal organoids 鈥 organ-like structures grown in the laboratory from stem cells that mimic some of the functions of native intestines 鈥 can potentially stimulate repair of the gut and recovery from NEC. The project will define how to best transplant organoids, identify how the organoids protect the intestine from injury and assess if organoid transplantation is a valid new treatment for NEC.

Lincoln Stein, who is head of adaptive oncology at the Ontario Institute for Cancer Research and a professor in the department of molecular genetics at 缅北强奸, has received seed funding to build a database called Cytomics Reactome, which will be freely available to Canadian and international researchers. The database will build on recent technologies that open the door to the possibility of deciphering cell lineage paths 鈥 the series of steps that lead a young, undifferentiated cell into a specialized one 鈥 at single-cell resolution. To accelerate the path from basic research to clinical application, the database will systematically organize pre-existing knowledge of cell lineage paths into a comprehensive, interactive and easily accessible map that can serve as a framework for interpretation and integration of the latest experimental findings.

Medicine by Design